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Monday, October 12, 2020 | History

3 edition of Microtubule protocols found in the catalog.

Microtubule protocols

Microtubule protocols

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Published by Humana Press in Totowa, NJ .
Written in English


Edition Notes

Statementedited by Jun Zhou.
Classifications
LC ClassificationsQH
The Physical Object
Paginationx, 305 p. :
Number of Pages305
ID Numbers
Open LibraryOL22763956M
ISBN 109781588296429

Tubulin inhibitors are chemotherapy drugs that interfere directly with the tubulin system, which is in contrast to those chemotherapy drugs acting on DNA. Microtubules play an important role in eukaryotic - and beta-tubulin, the main components of microtubules, have gained considerable interest because of their function and biophysical properties and has become the subject of. Learn microtubule with free interactive flashcards. Choose from different sets of microtubule flashcards on Quizlet.

Of course, the selection of protocols cannot be comprehensive, but the book covers a large variety of techniques. It starts with six protocols for how to get kinesin protein in your hands. Kinesin purification procedures from native and recombinant sources as well as strategies for the discovery of novel representatives from various organisms Author: Guenther Woehlke. Kinesin-like proteins (KLP) are thought to be responsible for transport of cellular cargoes and involved in many cellular processes. This book presents reproducible techniques for identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors.

The microtubule-associated protein TPX2 is a key mitotic regulator that contributes through distinct pathways to spindle assembly. A well-characterised function of TPX2 is the activation, stabilisation and spindle localisation of the Aurora-A kinase. High levels of TPX2 are reported in tumours and the effects of its overexpression have been. This volume in the popular Methods in Molecular Biology(TM) series offers an up-to-date collection of methods and approaches for investigating microtubule dynamics in vitro and in cells. Includes material lists, step-by-step protocols and troubleshooting s in Molecular Biology: Microtubule Dynamics: Methods and Protocols (Hardcover).


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Microtubule protocols Download PDF EPUB FB2

Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs.

The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident Format: Hardcover. This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs.

The book contains a straightforward presentation of readily reproducible protocols, tips for troubleshooting, and advice on avoiding common : $ About this book Introduction Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs.

Get this from a library. Microtubule protocols. [Jun Zhou;] -- "Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells.

Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin Format: Hardcover.

Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for.

In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin Brand: Humana Press.

item 3 Microtubule Protocols (English) Hardcover Book Free Shipping. - Microtubule Protocols (English) Hardcover Book Free Shipping. $ Free shipping. No ratings or reviews yet. Be the first to write a review. Best Selling in Nonfiction. See all.

In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells.

Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs.

The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident.

Get this from a library. Microtubule protocols. [Jun Zhou;] -- Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration.

Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to eukaryotic ubules can grow as long as 50 micrometres and are highly dynamic.

The outer diameter of a microtubule is between 23 and 27 nm while the inner diameter is between 11 and 15 nm.

They are formed by the polymerization of a dimer of two globular proteins, alpha and beta. How to Cite. Waterman-Storer, C. Microtubule/Organelle Motility Assays.

Current Protocols in Cell Biology. – By the end of the s only two microtubule-dependent motors, the plus end-directed kinesin and the minus end-directed cytoplasmic dynein, had been identified. At the time, these two motors seemed almost sufficient to explain directional motility events on polar microtubule tracks in the cell.

No- theless, shortly after, the tip of the iceberg. The NOOK Book (eBook) of the Microtubules, in vitro by John J. Correia at Barnes & Noble. FREE Shipping on $35 or more. Get FREE SHIPPING on Orders of $35+ Customer information on COVID B&N Outlet Membership Educators Gift Cards Stores & Events HelpPages: Preparation of Segmented and Polarity Marked Microtubules: 63 KB: Microtubule Spindowns from Extracts: 56 KB: Microtubule Spindowns for Visual Analysis: 51 KB: Large Scale Tubulin Preparation: 73 KB: Labeling Tubulin and Quantifying Labeling Stoichiometry: 83 KB: Flow Cell Assays with Microtubules: 76 KB: CHO Centrosome Prep: 68 KB.

Kinesin Protocols (methods In Molecular Biology) Download. By the end of the s only two microtubule-dependent motors, the plus end-directed kinesin and the minus end-directed cytoplasmic dynein, had been identified. Microtubules, the third principal component of the cytoskeleton, are rigid hollow rods approximately 25 nm in diameter.

Like actin filaments, microtubules are dynamic structures that undergo continual assembly and disassembly within the cell.

They function both to determine cell shape and in a variety of cell movements, including some forms of cell locomotion, the intracellular transport of. Microtubules are key components of the cytoskeleton, are composed of α-tubulin and β-tubulin and dimerize in a head to tail fashion to form linear protofilaments that associate initially into sheets and subsequently into hollow tubeswith a diameter of roughly 25 nm (1).

Each Microtubule is composed of 13 protofilaments of α-tubulin and β-tubulin heterodimers. Microtubule protocols (Book, ) [] Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to the cytoplasm of eukaryotic cells, some bacteria and some archaea (like Asgard).

A microtubule can grow as long as 50 micrometres and are highly dynamic. Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization Andreas Holzinger 5. Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts Christiane Wiese, Jonathan R.

Mayers, and Alison J. Albee Part III: Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components 6.ISOLATION OF MICROTUBULES. All eukaryotic cells assemble microtubules, as they are the main structural components of the mitotic apparatus.

However, the richest source of tubulin is terminally differentiated—and hence nondividing—neuronal tissue, because the axons and dendrites of neurons are full of assembled microtubules that can be readily depolymerized by cold by: 1.Tubulin Protocols (Mitchison Lab) CBG Protocol for recycling already in old protocol book.

GMPCPP stability within microtubule lattices, it is better to always use potassium counterion buffers for all our microtubule work. GMPCPP is a potent nucleator of microtubules. Therefore, at tubulin concentrations of 1 mg/ml or higher, veryFile Size: KB.